Fig. 3.

Blood loss in FIX ko mice after treatment with different doses of BAX326 or buffer. Four doses of BAX326 were tested to obtain a dose–effect curve. Buffer-treated animals served as negative controls and showed a median total blood loss of 1,036 mg. Median blood loss was reduced to 927 mg by treatment with 10 IU/kg of BAX326 and to 204 mg by treatment with 25 IU/kg rFIX. Treatment with 75 IU/kg rFIX, the dose also used in the carotid occlusion and TEG studies, led to a median blood loss of 145 mg, which was shown to be statistically different from buffer (P = 0.0085). Treatment with 100 IU/kg further reduced median blood loss to 32 mg. Statistical evaluation proved a monotone dose–response relation (P = 0.0076). The higher inter-individual variation observed for 75 IU/kg than for 25 IU/kg BAX326 is consistent with published results [13–15] and a known and accepted limitation of the model