Figure 1.

Characterization of five fibrillar α-Syn polymorphs. (A) Transmission electron micrographs of negatively stained α-Syn fibrillar polymorphs fibrils, ribbons, fibrils-91, fibrils-65, and fibrils-110 before (upper lane) and after fragmentation (lower lane) are shown. (B) Length distribution of the fragmented fibrillar polymorphs is shown. The number (n) of fibrillar assemblies the histograms were derived from is indicated. (C) Proteinase K degradation patterns of α-Syn (100 μM monomer concentration) polymorphs fibrils, ribbons, fibrils-91, fibrils-65, and the fibrillar form of the truncated form of α-Syn spanning residues 1–110 monitored over time on Coomassie-stained SDS-PAGE (12%) are shown. Time (min) and molecular weight markers (kDa) are shown on the top and left of each gel, respectively.