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. 2020 Jan 28;118(6):1301–1320. doi: 10.1016/j.bpj.2020.01.022

Figure 6.

Figure 6

Seeded pS129-α-Syn are composed of multiple intertwined elongated structures. (A–C) Super-resolution STORM imaging of endogenous pS129-α-Syn was performed at DIV 21 after exposure of primary neuronal cultures to the fibrillar α-Syn polymorphs fibrils, ribbons, and fibrils-91 (250 nM) for 15 min on DIV 14. Single-molecule detections for 40,000 frames, rendered with a pixel size of 10 nm, are shown for fibrils (A), ribbons (B), and fibrils-91 (C). In all three cases, elongated structures that seem to intertwine into larger bundles are seen. (D) The time course of elongated and intertwined pS129-α-Syn structure formation imaged by STORM after exposure of primary neurons to α-Syn fibrillar polymorph fibrils-91 after 2, 3, and 6 days is shown. (E and F) Quantitative analysis of the amount of elongated and intertwined pS129-α-Syn structures forming in primary neurons exposed to fibrillar α-Syn polymorphs (250 nM for 15 min on DIV 14) as a function of time is shown. Detection events within (E) and area occupied by (F) pS129-α-Syn aggregates are shown. The box plot shows median, interquartile range, and 10–90% distribution. A Mann-Whitney test was performed to compare the distributions; n is number of pS129-α-Syn aggregates analyzed: 40 for fibrils, 52 for ribbons, 56 for fibrils-91. ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05. Scale bars, 500 nm.