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. 2020 Jan 28;118(6):1301–1320. doi: 10.1016/j.bpj.2020.01.022

Figure 8.

Figure 8

Differential redistribution of synaptic α3-NKA, GluA2-AMPA, and GluN2B-NMDA in primary neurons exposed to distinct α-Syn fibrillar polymorphs. (A–J) Immunocytochemistry on DIV 21 of (A) α3-NKA (red) and Homer (green), (C) GluA2-AMPA receptors (red) and PSD95 (green), (E) GluN2B-NMDA receptors (red) and Homer (green) (G), GluA1-AMPA receptors (red) and PSD95 (green), and (I) mGluR5 receptors (red) and Homer (green). Primary neurons were exposed (15 min, 250 nM) to the fibrillar α-Syn polymorphs fibrils, ribbons, and fibrils-91 on DIV 14. Quantification of intensity of synaptic α3-NKA (B), GluA2-AMPA receptors (D), GluN2B-NMDA receptors (F), GluA1-AMPA receptors (H), and mGluR5 (J) clusters (indicative of size; see Material and Methods) was performed and plotted. The box plot shows median, interquartile range, and 10–90% distribution. Number of images (n) analyzed and plotted: (B) 47 from four independent experiments, (D) 37 from three independent experiments, (F) 30 from three independent experiments, (H) 37 from three independent experiments, and (J) 20 from two independent experiments. A Mann-Whitney test was performed. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ns, not significant. Dot plot shows the averaged value per experiment. Scale bars, 5 μM.