Table 1.
Antibodies Used for Immunolabeling and Quantification
| Primary Antibody | Host | Supplier | Fixation Protocol | Dilution |
|---|---|---|---|---|
| Organotypic Slices | ||||
| pS129-α-Syn (81A) | mouse monoclonal | Millipore MABN826 | 4% PFA 4°C 45 min |
1:1200 |
| p62/sequestosome-1 | rabbit polyclonal | Proteintech 55274-1-AP | 1:1000 | |
| NeuN (neurons) | mouse monoclonal | Millipore MAB377 | 1:800 | |
| Iba1 | rabbit polyclonal | Wako | 1:1000 | |
| Olig2 | rabbit polyclonal | Millipore AB9610 | 1:500 | |
| Primary Neurons | ||||
| pS129-α-Syn (81A) | mouse monoclonal | Millipore MABN826 | 4% PFA room temperature 10 min |
1:1200 |
| p62/sequestosome-1 | rabbit polyclonal | Abcam ab51253 | 1:1000 | |
| τ (Axons) | rabbit polyclonal | Synaptic System 314003 | 1:1200 | |
| α3-NKA | mouse monoclonal | Thermo Fisher Scientific MA3-915 | 1:800 | |
| mGluR5 | rabbit polyclonal | Millipore AB5675 | 1:1200 | |
| vGluT1 | guinea pig polyclonal | Millipore AB5905 | 1:1000 | |
| GluA1-AMPA | rabbit polyclonal | Synaptic System 182003 | 1:1200 | |
| GluA2-AMPA | rabbit polyclonal | Synaptic System 182103 | Methanol −20°C 10 min |
1:1200 |
| GluN2B-NMDA | mouse monoclonal | NeuroMab 75-101 | 1:200 | |