Figure 4.

Dependence of g10F6-binding antibodies on immunization regimen. (a) ELISA IgG EC₅₀ titers for rabbit serum binding to g10F6–BSA after three cycles of immunization with g10F6–CRM. Geometric means and standard deviations are shown. Numbers displayed above data are p-values determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons, using log-transformed data; ns denotes p > 0.1. (b) Titers as in panle a but plotted versus dose. All data except the prebleed are for sera collected 1 week after the dose: * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.001, **** denotes p < 0.0001. The dotted line indicates the lowest serum dilution tested in the ELISA.