Figure 7.

Carbohydrate selectivity of rabbit serum IgGs elicited by g10F6–CRM. (a–c) ELISA EC₅₀ IgG titers to different glycans displayed on peptide 10F12M–BSA for animals immunized by (a) group 1 (bolus), (b) group 2 (exponential), and (c) group 3 (pump) regimens. (d) Control ELISA of monoclonal macaque antibody DH501 binding to sugar conjugates from panels a–c. DH501 binds to (Manα1→2Man) termini of glycans, and unlike g10F6–CRM-elicited sera, binds identically to Man₉Cy and Man₉GlcNAc₂ conjugates. (e, f) For each rabbit, ratio of serum binding to full vs truncated glycan and full Man₉ glycan with vaccine-derived cyclohexyl linker vs GlcNAc₂. Numbers displayed above data are p-values determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons, using log-transformed data; ns denotes p > 0.1. For background binding of rabbit sera to BSA and linker, see Figure S5.