Figure 3.

The lysosome-associated membrane protein (LAMP) chimera enhances the antigen-specific cytokine responses. Mice were injected twice (a-1–a-3) and three times (b-1–b-4) with 50 μg of DNA plasmid. Antigen-specific splenocyte cytokine secretions were detected by enzyme-linked immunospot assay. The splenocytes were stimulated with overlapping peptides spanning the SARS-N epitope cluster region, amino acids 70–122, and with a yellow fever virus (YFV) peptide as a negative control. Each result was performed in three times individual experiment.