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. 2017 Apr 7;9(4):e371. doi: 10.1038/am.2016.128

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Illustration of tetrahedron-shaped polypeptide structure. (b) Coil-forming elements in the polypeptide self-assembly are denoted by arrow marks. Transmission electron microscopy (TEM) image of TET12 tetrahedral polypeptide highlighted in white boxes. TEM images of TET12 structure (left) stained with 1.8 nm Ni-NTA nanogold beads followed by uranyl acetate staining, scale bar=5 nm. (c) On the right, TEM images of TET12 polypeptide from the white boxes shown in (b)³⁷ (reprinted from Gradisar, H. et al. Design of a single-chain polypeptide tetrahedron assembled from coiled-coil segments. Nat. Chem. Biol. 9, 362–366. Copyright (2013), with permission from Macmillan Publishers Ltd.)