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In vivo gene transfer via sequential protocol is virus dose-dependent. Mice received tail vein injection of DOTAP:cholesterol liposomes (0.9 μmol DOTAP/mouse) followed by various amounts of AdCMVLuc. Gene expression in lung and liver was assayed 72 h following the injection (n = 7). *P < 0.05; **P < 0.01 (versus Ad alone).
