Figure 6.

Immunofluorescence detection of transgene-expressing cells. Mice received tail vein injection of DOTAP:cholesterol liposomes followed by AdCMVEGFP. Three days later mice were killed, lungs were collected, and cryosections were prepared. Immunofluorescence staining of PECAM in lungs was performed as described in Materials and methods. (a) Representative lung sections of mice receiving AdCMVEGFP. (b) Confocal microscopic images of lung sections of mice receiving AdCMVEGFP. (c) Lungs of control mice receiving AdCMVLuc. (d) Confocal microscopic images of lungs of control mice.