Skip to main content
. 2005 Apr 21;12(18):1394–1404. doi: 10.1038/sj.gt.3302535

Figure 6.

Figure 6

EGFR-specific coronavirus targeting. (a) NIH 3T3-hEGFR and NIH 3T3 cells were inoculated with FIPV or fMHV in the presence or absence of the bispecific adapter protein scFv 23F-425. After 1 h at 37°C, the inoculum was replaced by regular culture medium and incubation of the cells was continued. The cells were stained for coronavirus proteins 24 h after inoculation. Only NIH 3T3-hEGFR cells inoculated with FIPV or fMHV in the presence of the bispecific scFv 23F-425 stained positive. (b) Compared to EGFR-targeted infections of HeLa cells in the absence of blocking antibody, virus preincubation with anti-spike MAb 23F8.1, or preincubation of the cells with anti-EGFR MAb 425, decreased the amount of infected cells. The data represent the average percentage of infected cells relative to cells inoculated in the absence of blocking antibody. The error bars show the standard deviation of an experiment performed in triplicate.