Skip to main content
. 2014 Jan 28;5:3067. doi: 10.1038/ncomms4067

Figure 4. Biophysical analysis of the HR1P and HR2P peptides and their complex.

Figure 4

(a) Determination of the 6-HB formation between HR1P and HR2P by N-PAGE. The mixture of HR1P and HR2P at the final concentration of 35 μM was incubated at 25 °C for 30 min before being loaded into the gel. (b) Molecular mass determined by gel electrophoresis. Samples of each peptide with 50 μM were incubated, diluted 1:1 (v/v) with 2 × Laemmli sample buffer at room temperature and loaded into the gel. B, blank; M, marker. (c) Secondary structures of HR1P, HR2P and HR1P/HR2P complex in phosphate buffer: CD spectra for HR1P (10 μM), HR2P (10 μM) and their complex in phosphate buffer (pH 7.2) at 4 °C. (d) CD signal at 222 nm for the HR1P/HR2P complex as a function of temperature. Insert: curve of the first derivative (d[θ]/dT) against temperature (T), which was used to determine the Tm value.