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. 2014 Jan 28;5:3067. doi: 10.1038/ncomms4067

Figure 5. Images of MERS-CoV S protein-mediated cell–cell fusion and syncytium formation.

Figure 5

(a) MERS-CoV S protein-mediated cell–cell fusion. After Huh-7 cells were co-cultured with 293T/EGFP (left) or 293T/MERS/EGFP cells in the absence (middle) or presence (right) of 10 μM of HR2P at 37 °C for 4 h, cell–cell fusion was photographed under an optical microscope with fluorescence (top) or visible light (bottom). The arrows show the EGFP-labelled cells fused with unlabelled Huh-7 cells (top, middle). (b) MERS-CoV S protein-mediated syncytium formation. After Huh-7 cells were co-cultured with 293T/EGFP (left) or 293T/MERS/EGFP cells in the absence (middle) or presence (right) of 10 μM of HR2P, cell–cell fusion was photographed under an optical microscope with fluorescence or visible light. The arrows show the syncytia formed between 293T/MERS/EGFP and Huh-7 cells (middle). Scale bars, 200 μm.