Fig. 2.

a Mean arterial pressure (MAP), b low frequency (LF) of the systolic blood pressure (SBP), and c baroreflex function in rats fed either the standard diet (SD) or high-fat diet (HFD) before and after bilateral injections of losartan (LOS; 10 µg/100 nl) into the nucleus of the solitary tract (NTS). d Photomicrograph of a coronal section of the brainstem showing the typical location of the intermediate NTS microinjection sites. AP area postrema, CC central canal, X dorsal motor nucleus of the vagus, XII hypoglossal nucleus. Scale bar = 500 µm. The results are presented as the means ± SEM from the SD (n = 5) and HFD (n = 6) groups. Two-way ANOVA followed by the Student–Newman–Keuls test; *different from the SD group; #different from the HFD pre-LOS group; P < 0.05