Figure 3.
NC-inhibitory activity of compound 5–06 assessed by using the Nucleocapsid Annealing Mediated Electrophoresis (NAME) assay [24]. Briefly, an 8 μM aliquot of NC(12–55) was pre-incubated with increasing concentrations of 5–06 at room temperature for 15 minutes. Each sample was then added with 1 μM each of TAR29 and cTAR29, incubated at room temperature for 15 minutes, and then analyzed by non-denaturing polyacrylamide gel electrophoresis (see Materials and Methods for details).