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Regulatory effect of Egr-1 on HSV-1 promoters. HEK293 cells were co-transfected with pH4-2 and pJDM948. Lane 1: Cells tarnsfected with pH4-2 and the control plasmid (molar ratio, 1:1). Lane 2: Cells transfected with pH 4-2 and pJDM948 (molar ratio, 1:1). The cDNA of LAT, ICP4, ICP0, and actin were labeled. The quantitative analysis of RT-PCR signals was performed by Kodak Gel Logic 100 system.
