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. 2007 May 15;17(6):546–555. doi: 10.1038/cr.2007.44

Figure 5.

Figure 5

Regulation of ICP4 and ICP22 promoter activity by Egr-1 in the presence of TSA. (A) HEK293 cells were co-transfected with pICP22 or pICP22-ID and the control plasmid or pJDM948 in the presence (white bar) or absence (gray bar) of 100 nM TSA. 1, pICP22 co-transfected with control plasmid; 2, pICP22 co-transfected with pJDM948; 3, pICP22-ID co-transfected with control; 4, pICP22-ID co-transfected with pJDM948. The SEAP activity in lane 1 without TSA was arbitrarily set to 100 and the SEAP activity of others was normalized against this sample. The error bars represent SDs and the data were calculated and graphed using Microsoft Excel. (B) The effects of TSA on Egr-1 mediated regulation of ICP4 and ICP4-ID promoters were accessed using the strategy in (a) except that pICP4 and pICP4-ID were used instead.