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. 2007 May 15;17(6):546–555. doi: 10.1038/cr.2007.44

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© Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2007

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Co-repressor Nab2 was recruited to the ICP4 promoter in the presence of Egr-1 and Egr-1 significantly reduced the occupancy of Sp1 to the promoter when the intron was removed. HEK293 cells were co-transfected with the pICP4 (or pICP4-ID) and pJDM948. Lane 1, pICP4 and control; lane 2, pICP4 and pJDM948; lane 3, pICP4-ID and control plasmid, lane 4, pICP4-ID and pJDM948. The antibodies used for immunoprecipitation were labeled. Input control indicated the PCR products from samples prior to immunoprecipitation. Anti-Ig control confirmed the specificity of antibodies used in the experiments. The quantitative analysis was performed by Kodak Gel Logic 100 system. White bar: no Egr-1; gray bar: plus Egr-1.