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. 2020 Mar 24;17:13. doi: 10.1186/s12950-020-00242-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effect of UA on IL-1β-induced cartilage damage in cartilage explants culture. Rat cartilage explants were exposed to 30 ng/ml IL-1β alone or with 15 μM UA for 3 days. a. Representative H&E and S-O Fast Green staining revealed the IL-1β treated group had apparent morphological changes including rough surfaces (black arrow), clustered and disorganized chondrocytes (black triangle), obvious hypocellularity, and loss of Safranin-O staining compared with the control group (scale bar: 20 μm). b. Diagram showing the OARSI scores of the cartilage. c. Alcian Blue staining for glycosaminoglycan (GAG) distribution (scale bar: 20 μm). d. Immunohistochemical staining of Collagen II, Aggrecan expression in the cartilage samples (scale bar: 20 μm). e and f. The percentages of Collagen II and Aggrecan positive cells in each section were quantified by Image Pro Plus. Three sections were randomly selected for quantification, and original magnification were 40 × and 200× in overall and partial picture, respectively. Data are presented as mean ± S.D. n = 6. *P < 0.05, **P < 0.01 versus the IL-1β group