Fig. 5.

Drought tolerance analysis of the Gh4CL7-silencing cotton plants. a Representative phenotypes of the TRV:00 (CK) and TRV:Gh4CL7 (VIGS) plants after 3 weeks of drought treatment. b-c Leaf RWC and chlorophyll contents of CK and VIGS plants under drought stress. d-e Comparison of stomata in the CK and VIGS plants. f Water loss rate of detached leaves from the CK and VIGS plants. g-h Comparison of H₂O₂ and MDA contents in the CK and VIGS plants. i Activities of antioxidative enzymes in the CK and VIGS plants. Mock, normal growth conditions; drought, 3 weeks of water deficit conditions. Statistical significance was determined by the student test. * and ** represent significant at P < 0.05 and P < 0.01, respectively