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. 2020 Mar 23;20:125. doi: 10.1186/s12870-020-2329-2

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Drought tolerance analysis of the Gh4CL7-overexpressing Arabidopsis plants. a The relative expression level of Gh4CL7 in three independent Gh4CL7-OE Arabidopsis plants. b Germination rate of the Gh4CL7-OE Arabidopsis seeds on 1/2 MS supplemented with 0 and 300 mM mannitol. c-d Root elongation of the 6-days-old Gh4CL7-OE Arabidopsis seedlings on 1/2 MS supplemented with 0, 200, and 300 mM mannitol. Bar = 1 cm. e Phenotypic assay of the Gh4CL7-OE and WT Arabidopsis plants during drought treatment. f-h The contents of H₂O₂ and MDA and antioxidative enzyme activity in Gh4CL7-OE and WT plants under normal and water deficit conditions. Ten-days-old seedlings were transplanted to soil and regularly watered for 2 weeks. For drought treatment, irrigation was terminated for 2 weeks. For the rehydration treatment, the plants were re-watered 3 days after the drought treatment