Fig. 6.

Requirement of JNK activation in OM3.0 mediated changes in IAPP induced RINm5F cells. Cells were pretreated with OM3.0 and IAPP for 16 h and then with or without anisomycin as described. Protein isolates were subjected to a western blot analysis of JNK activation, b determination of complex III activity, and c assay of ATP content. Change in level of JNK phosphorylation (shown as ratio) was calculated based on level in corresponding untreated cells, which were set at unity. Values are mean ± S.E.M of the results from at least three independent experiments. ^##P < 0.01 versus control, *P < 0.05 versus the IAPP model, ^∇P < 0.05 versus the anisomycin treatment