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. Author manuscript; available in PMC: 2020 Mar 24.
Published in final edited form as: Cell Rep. 2019 Jun 25;27(13):3808–3817.e7. doi: 10.1016/j.celrep.2019.05.083

Figure 2. Dscam1-L Knockdown in Neurons Is Adult Lethal.

Figure 2.

(A) Top: schematic showing location of shRNA to knockdown Dscam1-L (shExt) using the GAL4-UAS system. Bottom: northern analysis using a probe for Dscam1 common exon 11 shows that shExt driven by Elav caused loss of Dscam1-L in dissected heads.

(B) Dscam1-L knockdown caused reduced survival in mixed sex adult flies. n = 6; 20 flies per replicate.

(C) Western analysis of Dscam1 normalized to α-tubulin shows no significant difference in shExt heads compared with control heads. n = 7. p value represents two-tailed unpaired Student’s t test.

Elav represents ELAV-Gal4, and Dicer2 represents UAS-Dicer2. See also Video S1.