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. Author manuscript; available in PMC: 2020 Mar 24.
Published in final edited form as: J Vis Exp. 2019 Mar 1;(145):10.3791/59233. doi: 10.3791/59233

Figure 2: Viral titering and colony screening.

Figure 2:

(A) An example p24 ELISA standard curve for lentivirus-containing, conditioned media. The black dots represent standard samples and the red dots unknown samples. (B) After transducing, selecting, and generating colonies, real-time PCR and gel electrophoresis against dCas9 were performed on the RNA from either nontransduced Jurkat cells (parental) or dCas9-transduced clones. No reverse transcriptase (No RT) controls were performed.