Skip to main content
. 2020 Mar 17;11:327. doi: 10.3389/fphar.2020.00327

Figure 4.

Figure 4

Effect of barttin overexpression on ClC-Kb wild type, G167V and A242E current amplitude. (A) Representative chloride current traces of ClC-Kb wild type, G167V, G289R, and A242E channels (5 µg) co-expressed with 15 µg barttin (1:3 ratio) in HEK 293 cells. (B) IV plot showing the mean current amplitude as a function of membrane potential of the indicated channels co-expressed with 15 µg barttin. (C) Chloride current measured at +60 mV as a function of the amount of co-expressed barttin for ClC-Kb WT, G167V, and A242E. Data are mean ± SE; n = 6–19. p < 0.05 for G167V (*) and A242E (#) compared with the respective ClC-Kb wild type. (D) Quantification of the surface expression efficiency for ClC-Kb and G167V channels co-expressed with 5 or 15 µg of barttin calculated by dividing surface protein density to the respective total protein density. The mean surface density of ClC-Kb and G167V channels co-expressed with 15 µg of barttin was normalized to the corresponding density of the same channels co-expressed with 5 µg of barttin. Data are mean ± SE of n = 3 experiments.