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No expression of ACE2 on neural cell lines derived from humans as well as from rats. (A) The OL and C6 cells were subjected to flow cytometry (upper panels) as well as membrane IF (lower panels) with anti-ACE2 PAb. (B) Total RNA extracted from the same cells used for (A) was subjected to quantitative RT-PCR for ACE2 mRNA using human ACE2 sequence-derived primers for Vero E6, CaCo-2, and OL cells and rat ACE2 sequence-derived primers for C6 cells.
