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. 2010 Jun 4;397(3):580–585. doi: 10.1016/j.bbrc.2010.05.161

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Copyright © 2010 Elsevier Inc. All rights reserved.

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Fig. 3 — Antibody responses and neutralizing titer detection of mAbs targeting HA of A/VietNam/1194/2004(H5N1) by pseudovirus neutralization assay. (A) ELISA detection of IgG response of 20 screened mAbs specific to HA. The data are expressed as absorbance at 450 nm (A450). (B) Pseudovirus neutralization detection of HA-specific mAbs against infection of HA pseudovirus in 293T cells. Ten mAbs with the highest antibody titer were selected for screening neutralizing activity. 33G4 mAb and VSV-G were used as negative mAb and pseudovirus controls, respectively. The data are presented as Mean NT₅₀ ± SD of 3 parallel wells of each dilution in 96-well culture plates. The dotted line indicates the detection limit. The experiment was repeated three times and similar results were obtained.