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. 2019 Oct 5;520(3):499–506. doi: 10.1016/j.bbrc.2019.09.115

Table 1.

Binding activity of packaging signal RNAs to the N-protein or deletion mutants by ONPG assay.

Protein Region (amino acid) LacZ Translation inhibition efficiency (%)
Binding Efficiency
MHV-PS97 hCoV-PS100
Full N 1–365 86 66 +++++
N1(45-181) 45–181 53 35 +++
N2(1-181) 1–181 83 59 +++++
N3(1-365) 1–365 80 63 +++++
N4(45-365) 45–365 53 56 ++++
N5(182-365) 182–365 19 14 +
N6(182-422) 182–422 32 25 ++
N7(249-365) 249–365 42 49 ++++