Table 1.
Primers for human VH and VL
| Human VH Back primers with XhoI | |
| VH1 Back XhoI | TTATCCTCGAGCGGTACCSAGGTSCAGCTGGTRCAGTCTGG |
| VH2 Back XhoI | TTATCCTCGAGCGGTACCCAGRTCACCTTGAAGGAGTCTG |
| VH3 Back XhoI | TTATCCTCGAGCGGTACCSAGGTGCAGCTGKTGSAG |
| VH4 Back XhoI | TTATCCTCGAGCGGTACCCAGSTRCAGCTRCAGSAGTS |
| Human VH For primers with NheI cite | |
| JH for NheI | GATTGGTTTGCCGCTAGCTGARGAGACRGTGACCRKKGT |
| Human Vκ Back primers with BssHII | |
| Vκ1 Back BssHII | AGCAAGCGGCGCGCATGCCGMCATCCRGDTGACCCAGTCTCC |
| Vκ2 Back BssHII | AGCAAGCGGCGCGCATGCCGATATTGTGMTGACBCAGWCTCC |
| Vκ3 Back BssHII | AGCAAGCGGCGCGCATGCCGAAATTGTGHTGACDCAGTCTCC |
| Vκ1 Back BssHII | AGCAAGCGGCGCGCATGCCGAAACKACACTCACGCAGTCTC |
| Human Vκ For primers with SalI | |
| Jκ1 for SalI | GAAGTTATGGTCGACCCTCCGGAACGTTTGATHTCCASYTTGGTCC |
| Jκ2 for SalI | GAAGTTATGGTCGACCCTCCGGAACGTTTAATCTCCAGTCGTGTCC |
| Human Vλ Back primers with BssHII | |
| Vλ1 Back BssHII | AGCAAGCGGCGCGCATGCCCAGYCWGYBYTGAYKCAGCC |
| Vλ2 Back BssHII | AGCAAGCGGCGCGCATGCCTCCTMTGWGCTGABDCAGS |
| Vλ3 Back BssHII | AGCAAGCGGCGCGCATGCCAATTTTATGCTGACTCAGCCCC |
| Human Vλ For primers with SalI | |
| Jλ for SalI | GAAGTTATGGTCGACCCTCCGGAACCTAGGACGGTSASCTTGGTCCC |
Note. A set of human antibody primers for amplification of the VH and VL genes from B cells of SRAS patients was optimized based on previous publications [20], [21], [27], [28], [29].
The italic indicates the flank sequence of the primers, the underline indicates the restriction enzyme site, and the bold indicates the primer sequence for amplifying the genes of human VH and VL.