Skip to main content
. 2005 Dec 9;340(1):158–164. doi: 10.1016/j.bbrc.2005.11.168

Fig. 4.

Fig. 4

Specificity of siRNA-mediated target gene silencing. COS-1 cells were cotransfected with plasmids expressing SPAG9 and JNK-3 with BS/U6 (A,B) or BS/U6/gfp (C,D) or BS/U6/spag9 (E,F), respectively. Representative photomicrographs of cells expressing SPAG9 (A, C, and E; SPAG9 expression probed with anti-SPAG9 antibody) and JNK-3 (B, D, and F; JNK-3 expression probed with anti-FLAG monoclonal antibody). SPAG9 expression was drastically reduced in the BS/U6/spag9 treated cells (E), whereas normal JNK-3 expression was observed in all the treated cells (B, D, and F) (original magnification 1000×).