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Semi-quantitative RT-PCR assay for cytokine gene expression. Total RNA was isolated from spleen of the immunized groups or normal mice (3 for each group). The levels of the cytokines IL-2, IFN-γ, IL-4, and IL-10 were semi-quantitatively measured by a competitive RT-PCR with the addition of the 150 pg/ml of the pQRS competitor after the normalization of tested cDNA concentration to a constant amount of HPRT as described in Materials and methods.
