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. 2005 Apr 19;331(4):1193–1200. doi: 10.1016/j.bbrc.2005.04.032

Fig. 2.

Fig. 2

SE-FPLC analysis of the 2-helix protein. The mixture of the 2-helix protein, BSA, and lysozyme was run on Superdex G75 gel-filtration. Inset was the Tris–Tricine SDS–PAGE result. Lanes 1, 2, and 3 are corresponding to the samples from the peaks 1, 2, and 3, respectively. Lane 4 is protein marker (kDa) and lane 5 is the purified 2-helix protein (11 kDa). The 2-helix protein (peak 2, 34.4 kDa) was eluted between BSA (peak 1, 67 kDa) and lysozyme (peak 3, 14.4 kDa) protein standards, which demonstrated that the 2-helix protein might form a trimer (3 × 11 = 33 kDa, closer to 34.4 kDa).