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EDANS/DABCYL-conjugated SARS N-protein peptide assay. EDANS/DABCYL-conjugated 181SQASSRSSSRSRGNSRNSTP200 peptide was generated as artificial protease substrates. Increased fluorescence (measured in relative fluorescence units, RFU) indicated proteolysis. Ten micrograms and 100 ng trypsin were used as a positive controls along with SARS N-protein samples from either M15 or BL21(DE3) cells.
