Fig. 7.

Chemical cross-linking of the TGEV fusion core with different concentrations of chemical cross-linker, EGS (ethyleneglycol-bis (succinimidyl succinate) from Pierce). (A) Cross-linked products were separated on 17% SDS–PAGE followed by Coomassie brilliant blue staining. Protein marker (M) is shown in kDa. Lanes 1–6 indicate the different concentrations of the EGS used (0, 0.2, 0.5, 1.0, 1.5, and 2.0 mM, respectively). Bands corresponding to monomer, dimer, and trimer are indicated. (B) The cartoon depicting the schematic diagram of the cross-linking.