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. 2008 Jun 30;373(4):561–566. doi: 10.1016/j.bbrc.2008.06.078

Fig. 3.

Fig. 3

DC-SIGN mediates trans infection by H5N1 pseudotyped and reverse-genetics viruses. (A) The both sets of donor cells B-THP-1, B-THP-1/DC-SIGN and THP-1, THP-1/DC-SIGN were used in capture assay. The bars represent luciferase activity measured in MDCK cell lysates following co-culturing with donor cells in the presence or absence of MAb. (B) Immature and mature DCs were infected with H5N1 pseudotyped viruses for 0–48 h and cell morphology was observed under a light microscope. Upper and lower panels indicate iDCs and mDCs, respectively. (C) Immature and mature DCs with or without anti-DC-SIGN MAb treatment were used as donor cells to capture H5N1 pseudotyped virus particles and for co-culturing with MDCK cells. Luciferase activity was determined in MDCK lysates 48 h post-treatment. (D) The H5N1 reverse genetics strain A/Vietnam/1194/04 (105 copies/ml) was used for a capture assay. Virus-bound sialidase-treated B-THP-1/DC-SIGN and B-THP-1 donor cells were co-cultured with MDCK cells for 48 h; real-time RT-PCR was used to quantify H5N1 viral RNA in MDCK cell lysates. Results shown are from one representative experiment out of three performed (∗p < 0.05; ∗∗p < 0.01).