Fig. 2.
The antigenic regions of broadly neutralizing HuMAbs. (A) Based on the reactivity of B-1 and D-1 HuMAbs with a series of overlapping peptides covering sequences of the HA1 region, amino acid sequences from aa 167–187 and from aa 225–241 are shown. Common amino acid sequences reactive with HA1 peptides are aa 173–181 and from aa 227–239 that are boxed by red and green, respectively. (B) The monomeric structure of H3 HA is shown. The image was created with RasMol 2.6.4, and the HA structure was obtained from the Protein Data Bank (PDB Accession No. 2VIU). The positions of the binding sites are shown as red dots (aa 173–181) and green dots (aa 227–239). The receptor-binding sites are shown as black ribbons. (C) The deduced amino acid sequences of CDRs in the VH and VL of HuMAbs B-1 and D-1 are shown.