Fig. 1.

Development of a new anti-ACE2 mouse mAb H8R64. (A) Lysates of HCC827 GR2 cells were immunoprecipitated with mAb H8R64. The band at 110 kDa clearly appears under reducing conditions. (B) Identification of the 110 kDa band as ACE2 using mass spectrometry. Boldface type indicates the sequence of the detected peptides. (C) Knockdown experiments using ACE2 siRNAs (10 nM each) confirm that the molecule mAb H8R64 recognizes is ACE2. (D) Viability of HCC827 GR2 cells that were transfected with NC siRNA or ACE2 siRNA (10 nM each), grown for 72 h, and then incubated with control IgG-DT3C conjugate or H8R64-DT3C conjugate for another 72 h. Results are presented as means ± SD from triplicate cultures. *P < 0.05.