Fig. 1.

mαSGT antibody detects human and Vero E6 SGT. (A) Mouse anti-SGT specifically detects endogenous SGT from Vero E6 cells (mSGT), as well as transfected flag-hSGT. (B) Co-immunoprecipitation of flag-hSGT and mSGT with 7a-HA. Lysates from Vero E6 cells transfected with flag-hSGT and/or 7a-HA were extracted and immunoprecipitated (IP) with rabbit anti-flag (lane 4), rabbit anti-SGT (lane 5) or unrelated rabbit antibody (lane 6) conjugated to Protein-A beads, respectively. IP samples (lanes 4–6), together with direct lysates (lanes 1–3) were analyzed by 15% SDS–PAGE and Western blotting with the appropriate mouse anti-SGT or mouse anti-HA antibody.