Fig. 1.

Depletion of cholesterol by MβCD inhibits PRRSV infection in MARC-145 cells. MARC-145 cells were pretreated with 0, 1, 2 and 3 mM MβCD for 1 h, followed by PRRSV infection in the presence of various concentrations of MβCD. After a 24 h infection period, the culture supernatants were harvested for viral titration using the TCID₅₀ assay (A), and the PRRSV infection cells were assayed by IFA using monoclonal antibody against N protein (green) (B). Cell viability was determined using MTT assay (C) and cholesterol levels were measured with Amplex Red cholesterol assay kit (D). The averages from three independent experiments with standard deviation error bars are shown. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this paper.)