Fig. 2.

Depletion of cholesterol by MβCD affects PRRSV entry, but has no effect on binding in MARC-145 cells. For binding assays, MARC-145 cells were pretreated with 0, 5, 10 and 20 mM MβCD for 1 h, and then were inoculated with PRRSV at an MOI of 1.0 at 4 °C. After 90 min, the infection was either analyzed by FACS analysis using anti-N antibodies (A) or real-time PCR analysis of the PRRSV genome (B). For entry studies, the cells were shifted to 37 °C followed by washing with PBS. After 24 h, virus titer and virus reverse transcription products were detected using TCID₅₀ (C) and real-time PCR (D), respectively. Data represent mean ± SD of results obtained with three independent experiments.