Fig. 3.

Exogenous cholesterol can partially restore virus infection. MARC-145 cells pretreated with 10 mM MβCD were supplemented with 400 μg/ml cholesterol before PRRSV infection. After infection for 24 h, viral titers were measured by TCID₅₀ using the Reed–Muench method (A) and the copy number of PRRSV genome was analyzed using real-time PCR (B). Mock treated and MβCD treated without cholesterol supplemented cells were used as controls. Experiments were repeated three times, error bars represent deviation of the median from three experiments.