Supplementary Fig. 1.

MARC-145 cells were treated with 0, 5, 10, 20 mM MβCD for 24 h. Cell viability was determined using MTT assay (A) and cholesterol levels were measured with Amplex Red cholesterol assay kit (B). For PRRSV infection, MARC-145 cells infected with PRRSV for 6 h, and then treated with 0, 5, 10, 20 mM MβCD for 1 h. The virus genome was determined by real-time PCR(C) and the supernatants were harvested for viral titration using TCID₅₀ assay after culturing another 18 h (D). Data represent mean ± SD of results obtained with three independent experiments.