Supplementary Fig. 2.

Filipin inhibit PRRSV entry into MARC-145 cells. (A) Cell viability was determined in the mock-infected MARC-145 cells treated with filipin (0, 0.5, 1, 2 μg/ml) for 1 h. (B and C) MARC-145 cells were pretreated with 0, 0.5, 1, 2 μg/ml filipin for 1 h, and then were inoculated with PRRSV at MOI of 1 at 4 °C. Ninety minutes later, the cells were shifted to 37 °C followed by washing with PBS. After incubation in 37 °C for 24 h, virus reverse transcription products and virus titer were detected using real-time PCR (B) TCID₅₀ (C), respectively. Data represent mean ± SD of results obtained with three independent experiments.