Supplementary Fig. 3.

Mevinolin inhibit PRRSV entry into MARC-145 cells. (A) Cell viability and the effect of cholesterol level (B) were determined in the mock-infected MARC-145 cells treated with mevinolin (0, 1, 2, 4 μM) for 48 h. (C and D) MARC-145 cells were pretreated with 0, 1, 2, 4 μM mevinolin for 48 h, and then were inoculated with PRRSV at MOI of 1 at 4 °C. Ninety minutes later, the cells were shifted to 37 °C followed by washing with PBS. After incubation in 37 °C for 24 h, virus titer and virus reverse transcription products were detected using TCID₅₀ (C) and real-time PCR (D), respectively. Data represent mean ± SD of results obtained with three independent experiments.