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The primer sequences used for real-time PCR.
| Gene | Primer sequence (5′ to 3′) | |
|---|---|---|
| ORF7 | Forward | TTGTGTCTGTCGTCGATCCAG |
| Reverse | AAACTCCACAGTGTAACTTATCCTC | |
| Probe | (FAM) CGCTGGAACTTGTGCCCTGTCA (Eclipse) | |
| β-Actin | Forward | TGACTGACTACCTCATGAAGATCC |
| Reverse | TCTCCTTAATGTCACGCACGATT | |
| Probe | (FAM) CGGCTACAGCTTCACCACCACGGC (Eclipse) | |
