Table 1.

The sequences of the oligonucleotides used in this study

31-mer thrombin-inhibiting aptamer	5′ CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG 3′
AES 1	5′ CACTGGTAGGTTGGTGTGGTGCGAGAAGTTAAGACCTATGCTCGCTGGGGCCAGTG 3′
AES 2	5′ CACTGGTAGGTTGGTGTGGTTGCGAGAAGTTAAGACCTATGCTCGCTTGGGGCCAGTG 3′
15-mer target DNA	5′ CATAGGTCTTAACTT 3′
15-mer control DNA	5′ ATTGCTATCGTACAT 3′
AES SARS 1	5′ CACTGGTAGGTTGGTGTGGTTGGACGACGAATTCATGATCACGTCCTTGGGGCCAGTG 3′
15-mer SARS DNA	5′ TGATCATGAATTCGT 3′
Stem and loop SARS 1	5′TGGACGACGAATTCATGATCACGTCCT 3′
AES SARS 2	5′ CACTGGTAGGTTGGTGTGGTTCGGACGCACGAATTCATGATCAACATCCCTACGTCCGTTGGGGCCAGTG 3′
AES SARS 3	5′ CACTGGTAGGTTGGTGTGGTTGCGGACGCACGAATTCATGATCAACATCCCTACGTCCGCTTGGGGCCAGTG 3′
AES SARS 4	5′ CACTGGTAGGTTGGTGTGGTTCGCGGACGCACGAATTCATGATCAACATCCCTACGTCCGCGTTGGGGCCAGTG 3′
25-mer SARS DNA	5′ TAGGGATGTTGATCATGAATTCGTG 3′

The stem-and-loop sequence inserted into the 31-mer thrombin-inhibiting aptamer is underlined. The 15-mer target DNA, the 15-mer SARS, and the 25-mer SARS are complementary strands of the loop sequence of AES 1, 2, and AES SARS 1 and AES SARS 2, 3, and 4, respectively. The stem-and-loop SARS 1 sequence is the oligonucleotide inserted into AES SARS 1.