Fig. 3.

Reduced SARS-CoV spike transcripts in S-specific siRNA transfected HEK 293T cells. (A) RT-PCR was performed to show the degradation of S mRNA in S-specific siRNA transfected cells. RT-PCR products for the spike gene and the internal control, GAPDH, are shown in the figure. (B) Real-time quantitative PCR was performed for spike and GAPDH RNA content on each sample. The assay shows the percentage of SARS-CoV spike mRNA in siRNA transfected HEK 293T cells relative to that in mock-transfected cells after being normalized to GAPDH mRNA.