Fig. 4.

Effect of siRNAs on the expression of S-EGFP in HEK 293T cells. (A) HEK 293T cells. (B) pEGFP-optS transfected. (C) pEGFP-optS and EGFP siRNA co-transfected. (D) pEGFP-optS and scramble siRNA co-transfected. (E) pEGFP-optS and S-siRNA1 co-transfected. (F) pEGFP-optS and S-siRNA2 co-transfected. Upper panels represent the cell fluorescence images recorded at 48 h posttransfection. Lower panels represent the light microscopic view of cells in the same field. Specific silencing of the S-EGFP fusion protein expression was confirmed in three independent experiments.