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. 2004 Oct 21;324(4):1186–1193. doi: 10.1016/j.bbrc.2004.09.180

Table 1.

Sequences of template deoxynucleotides for siRNAs used for target genes

Gene targets Sequences
T7 promoter 5′-TAATACGACTCACTATAGGAGACAGG-3′
EGFP-siRNA Antisense: 5′-AAGCTGACCCTGAAGTTCATCCCTGTCTC-3′
Sense: 5′-AAGATGAACTTCAGGGTCAGCCCTGTCTC-3′
Scramble siRNA Antisense: 5′-AACAAGTCTCGTATGTAGTGGCCTGTCTC-3′
Sense: 5′-AACCACTACATACGAGACTTGCCTGTCTC-3′
S-siRNA1 Antisense: 5′-AAGAGCTTTGAGATTGACAAACCTGTCTC-3′
Sense: 5′-AATTTGTCAATCTCAAAGCTCCCTGTCTC-3′
S-siRNA2 Antisense: 5′-AACCCTTTCTTTGCTGTTTCTCCTGTCTC-3′
Sense: 5′-AAAGAAACAGCAAAGAAAGGGCCTGTCTC-3′

Note. The 8 nucleotide (nt) underlined sequences of template deoxynucleotides for each siRNA are complemented with those of T7 promoter primer. S-siRNA1 and S-siRNA2 target nt 403–423 and nt 871–891 of SARS-CoV spike gene, respectively.