Table 1.
Gene targets | Sequences |
---|---|
T7 promoter | 5′-TAATACGACTCACTATAGGAGACAGG-3′ |
EGFP-siRNA | Antisense: 5′-AAGCTGACCCTGAAGTTCATCCCTGTCTC-3′ |
Sense: 5′-AAGATGAACTTCAGGGTCAGCCCTGTCTC-3′ | |
Scramble siRNA | Antisense: 5′-AACAAGTCTCGTATGTAGTGGCCTGTCTC-3′ |
Sense: 5′-AACCACTACATACGAGACTTGCCTGTCTC-3′ | |
S-siRNA1 | Antisense: 5′-AAGAGCTTTGAGATTGACAAACCTGTCTC-3′ |
Sense: 5′-AATTTGTCAATCTCAAAGCTCCCTGTCTC-3′ | |
S-siRNA2 | Antisense: 5′-AACCCTTTCTTTGCTGTTTCTCCTGTCTC-3′ |
Sense: 5′-AAAGAAACAGCAAAGAAAGGGCCTGTCTC-3′ |
Note. The 8 nucleotide (nt) underlined sequences of template deoxynucleotides for each siRNA are complemented with those of T7 promoter primer. S-siRNA1 and S-siRNA2 target nt 403–423 and nt 871–891 of SARS-CoV spike gene, respectively.